IsoAmp® III Enzyme Mix is used to perform thermophilic Helicase-Dependent Amplification (tHDA), a novel method for isothermal amplification of nucleic acids. Like PCR, the tHDA reaction selectively amplifies a target sequence defined by two primers. However, unlike PCR, tHDA uses an enzyme called a helicase to separate DNA, rather than heat. This allows DNA amplification without the need for thermocycling. The tHDA reaction can also be coupled with reverse transcription for RNA analysis.
IsoAmp® III Enzyme Mix contains helicase, DNA polymerase and single-stranded DNA binding protein in a carefully optimized condition to support robust and specific amplification with reduced non-specific products due to primer-dimer. In some cases, rapid amplification may be achieved by adding more IsoAmp® III Enzyme Mix into the reaction (unpublished observation).