IsoAmpIII Universal tHDA kit can be used to amplify and detect short
DNA sequences (70 bp - 120 bp) at a constant temperature. The kit
can be used with a variety of templates, including microbial genomic
DNA, viral DNA, plasmid DNA, and cDNA. A single copy of target DNA
can be amplified by tHDA and detected by agarose gel electrophoresis
when optimized primers and buffer are used.
IsoAmp® III Universal tHDA kit is based on a third-generation tHDA formula for more robust and specific amplification with reduced non-specific products due to primer-dimer. In some cases, rapid amplification may be achieved by adding more IsoAmp® III Enzyme Mix into the reaction (unpublished observation). The volume of IsoAmp® III Enzyme Mix has been reduced to 2 µL per reaction, which allows the user to add more enzymes (2 X or 3 X) for possible high-speed amplification. IsoAmp® III Enzyme Mix (Cat# H1020S) can be purchased separately.
IsoAmp® III Universal tHDA kit supports all reactions which are supported by IsoAmp® II Universal tHDA kit. Selected examples are included in this manual, including end-point tHDA and real-time quantitative HDA (qHDA) in both one-step and two-step formats. A Control Template and a set of specific primers are supplied for the positive control reaction.