IsoAmpII Universal tHDA kit can be used to amplify and detect short
DNA sequences (70 bp - 120 bp) at a constant temperature. The kit
can be used with a variety of templates, including microbial genomic
DNA, viral DNA, plasmid DNA, and cDNA. A single copy of target DNA
can be amplified by tHDA and detected by agarose gel electrophoresis
when optimized primers and buffer are used.
IsoAmpII Universal tHDA kit is based on a second-generation thermophilic
Helicase-Dependent Amplification platform. The reactions supported
by IsoAmp® II Universal tHDA kit include tHDA, reverse transcription
HDA (RT-HDA), real-time quantitative HDA (qHDA) and real-time quantitative
RT-HDA (qRT-HDA), from a single reaction buffer.
IsoAmpII Universal tHDA kit contains an enzyme mix,
dNTP solution and buffer, which allows flexibility in reaction setup.
A Control Template and a set of specific primers are supplied for
the positive control reaction.