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IsoAmp®II Universal tHDA Kit

 

Isothermal DNA Amplification - Research Use Only

Catalog #
Size
Concentration
Price
H0110S
50 reactions (50 µl reaction)
$298.00

Download: Manual ; MSDS

   

Description:

   


IsoAmpII Universal tHDA kit can be used to amplify and detect short DNA sequences (70 bp - 120 bp) at a constant temperature. The kit can be used with a variety of templates, including microbial genomic DNA, viral DNA, plasmid DNA, and cDNA. A single copy of target DNA can be amplified by tHDA and detected by agarose gel electrophoresis when optimized primers and buffer are used.

IsoAmpII Universal tHDA kit is based on a second-generation thermophilic Helicase-Dependent Amplification platform. The reactions supported by IsoAmp® II Universal tHDA kit include tHDA, reverse transcription HDA (RT-HDA), real-time quantitative HDA (qHDA) and real-time quantitative RT-HDA (qRT-HDA), from a single reaction buffer.

IsoAmpII Universal tHDA kit contains an enzyme mix, dNTP solution and buffer, which allows flexibility in reaction setup. A Control Template and a set of specific primers are supplied for the positive control reaction.

   



Figure 1. HDA Technology.

   


 


Figure 2.
Positive control assays with varying amount of Control Template.
Positive control assays were conducted following the one-step qHDA protocol. Control Template was used at 5 ng - 500 fg per assay in triplicate.

* Negative control reactions may produce primer-dimer, which can be distingushed from specific amplicons by gel-analysis or by melting-curve analysis.

 

Applications:

   


Used to amplify and detect short DNA sequences (80 - 120 bp)

- Download Manual

Advantages:
Use of helicase to separate DNA eliminates need for thermocycler
Reactions can be performed at constant temperature.
Works with a variety of templates, including bacterial genomic DNA, viral DNA, plasmid DNA and cDNA.
As low as a single copy of target DNA can be amplified under optimized conditions.
Can be used for real-time quantitative or/and reverse transcription HDA.

     

Kit Components:

   


IsoAmp® Enzyme Mix (50 reactions)
IsoAmp® dNTP Solution (50 reactions)
10 X Annealing Buffer II
MgSO4 (100 mM)
NaCl (500 mM)
pCNG1 (Control Template: 1 ng/µl)
NGF3 (Forward Primer: 5 µM)
NGR3 (Reverse Primer: 5 µM)

Note: Control Template and Primers have been changed.

   
     

Storage Conditions:

   


Storage Temperature:
-20ºC

Recommended storage conditions for the tHDA kit are -20°C for short-term (less than 6 months) storage and -80°C for storage greater than 6 months.
     
   

Quality Control:

 


Quality Assurance Statement:

Kit components were individually tested for optimal performance in tHDA.

     
   

Legal:

 

Licenses/Patents/Disclaimers:
NOTICE TO PURCHASER: This product is covered by U.S. patents 7,282,328 and 7,662,594 and pending foreign patents. The purchase price of this product includes a limited, non-transferable license for research use only under the patents owned by BioHelix. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of the product. The Product is not to be used for diagnostic purposes nor is it intended for human use. This product may not be resold, modified for resale, or used to manufacture commercial products without written approval of BioHelix Corp. Further information regarding this product may be obtained by contacting BioHelix at information@biohelix.com.

 
   
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