time (< 2 hours)
A 121 bp target fragment is amplified by isothermal Helicase
Dependent Amplification (HDA) using one common primer and two
allele specific primers: one for the wild type G (labeled with FI)
and the other for the mutant A (labeled with DIG). After amplification,
the reaction tube is placed in a Type II BESt cassette for
genotype determination. A Wild-type allele (W) will show a visible
T line and Mutant allele (M) will show a colored C line (Figure
1). Both T and C lines will be visible for a heterozygous genotype
(H). It is invalid if no visible line is observed.
Figure 1. Typical results on BESt Cassette.