The pWGA technology uses a primase to synthesize primers
on-site, generating multiple initiation sites for random, whole
genome amplification. Therefore, there is no need to add random
primers to the reaction.
The pWGA technology also utilizes a helicase to denature
double-stranded template DNA, eliminating the initial heating step
that is required by other WGA technologies. Thus, the Rapisome
pWGA reaction is truly isothermal.
pWGA is also faster than any other commercially available WGA techniques
: the reaction time for pWGA is less than one hour. This
makes Rapisome pWGA attractive technology for use in diagnostic
applications more so than other conventional methods.