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T7 Gp4A Helicase

 

Products - Helicases

Catalog #
Size
Concentration
Price
H0250S
50 µg
1 mg/ml
$568.00

Download: MSDS

   

Description:

   


The T7 Gp4A Helicase exhibits a 5’ to 3’ helicase activity as well as primase activity. It is highly processive, traveling an average of 75 kb of single stranded DNA before dissociating.

SUPPLIED WITH:
10X Gp4A Reaction Buffer

Supplied by Users: dTTP

     
     

Source:

   


Purified from an E. coli strain that overexpresses the gp4A gene isolated from the T7 phage.
     
     

Reaction condition:

   


1X Gp4A Reaction Buffer:
35 mM Tris–acetate, pH 7.5
11 mM magnesium acetate
5 mM DTT
0.01% Triton X-100

Supplement with 2 mM dTTP.
Incubate at 25 - 37ºC.

NOTE:

The concentration of dTTP should be optimized for each specific application.
For the primase activity, supplement reactions with NTPs.

 
     

Storage Conditions:

   


Storage Temperature:

-20ºC

Concentration:
1 mg/ml

Storage Buffer:

20 mM TrisHCl pH 7.5
100 mM NaCl
0.1 mM EDTA
1 mM DTT
50% Glycerol
pH 7.5 @ RT
     
     

Quality Control:

   


Quality Assurance Statement:

T7 Gp4A Helicase is purified free of contaminating endonucleases and exonucleases. Each lot is tested for DNA unwinding activity in functional activity assays.

     
   
   
 
 
 
   
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