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BioHelix has commercialized two isothermal DNA amplification technologies, HDA and pWGA. These novel amplification technologies depend on the DNA-unwinding activity of an enzyme called a Helicase. Helicase may be useful in a variety of applications, therefore we have made our helicases available to the research communuty for research use only.

E. coli UvrD Helicase
  • Isolated from a strain of E. coli overexpressing the UvrD helicase.
  • Unwinds double-stranded DNA.
  • Optimal temperature for activity: 37ºC
 
Thermostable DNA Helicase
  • Isolated from a strain of E. coli carrying the cloned helicase gene from a thermophilic bacterium.
  • Unwinds double-stranded DNA.
  • Optimal temperature for activity: 45 - 65ºC

T7 Gp4A Helicase

  • Isolated from a E. coli strain carrying the cloned gp4 gene from T7.
  • Highly processive replicative helicase that forms a hexameric ring.
  • Active at a temperature ranging from 25 to 37ºC.


         
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